The game will take place at The Hive on Sunday 1st October (Kick-off time TBC).
London Bees are one league above Worthing, in the Southern Premier Division and finished 9th in the league last season, but have lost their opening four league fixtures this term.
The Bees overcame London Seaward in the Determining Round, with a comfortable 3-1 win.
A first-half hat-trick from Sammy Quayle saw Worthing safely through from their Determining Round tie against Portishead in the League Cup.
Kick-off time and ticket information will be confirmed in due course.